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Anion exchange (AEX) and cation exchange (CEX) chromatography methods were established, as described in a previous work (Supplementary Figure S7C)

Diet-induced obese animals exhibit indices of altered central monoaminergic neurotransmission, including reduced hypothalamic 1 adrenoceptor binding, enhanced electrophysiological responsiveness to norepinephrine application, decreased basal hypothalamic serotonin and dopamine levels, and reduced mesolimbic dopamine activity

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